US 12,297,227 B2
Method for extracting target protein from biological sample and method for analyzing target protein
Shin-ichiro Nitta, Tokyo (JP)
Assigned to Mediford Corporation, Tokyo (JP)
Appl. No. 16/618,787
Filed by Mediford Corporation, Tokyo (JP)
PCT Filed Jun. 1, 2018, PCT No. PCT/JP2018/021292
§ 371(c)(1), (2) Date Dec. 2, 2019,
PCT Pub. No. WO2018/221745, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 2017-110247 (JP), filed on Jun. 2, 2017.
Prior Publication US 2020/0140482 A1, May 7, 2020
Int. Cl. C07K 1/14 (2006.01); G01N 1/40 (2006.01); G01N 21/64 (2006.01)
CPC C07K 1/145 (2013.01) [G01N 1/4044 (2013.01); G01N 21/64 (2013.01)] 10 Claims
 
1. A method of extracting one or more target protein(s) from a sample derived from a living body by a deproteinization method, comprising:
extracting said one or more target protein(s) by using a salt at a concentration of 0.02 to 8 mol/L, and a water-soluble organic solvent, wherein
said extracting comprises:
adding the salt at a concentration of 0.02 to 8 mol/L to the sample derived from the living body with stirring:
adding the water-soluble organic solvent to the sample to which the salt have been added, wherein an amount of the added water-soluble organic solvent is twice or more (v/v) an amount of the sample derived from the living body;
separating the sample to which the water-soluble organic solvent has been added; and
collecting a supernatant comprising said one or more target protein(s) after the separation,
wherein the sample is selected from the group consisting of blood, plasma, serum, urine, stool, saliva, sputum, sperm; secretion, excreta, or swab from vagina, nose, rectum, urethra, or pharynx; secretory fluid from lacrimal duct, bronchoalveolar lavage fluid (BALF), pleural effusion, and a biopsy tissue sample,
wherein said one or more target protein(s) is a protein being able to be dissolved in a water-soluble organic solvent, and wherein said one or more target protein(s) has a molecular weight of 50,000 Da or less.