US 12,296,334 B2
Microfluidic devices and arrangements for supplying such devices with reagents and biological samples
Jeffrey Kenneth Horton, Cardiff (GB); Simon Laurence John Stubbs, Cardiff (GB); Peter James Tatnell, Cardiff (GB); Geraint Seymour, Cardiff (GB); Cheryl Louise Potts, Cardiff (GB); Alan Stuart Pierce, Cardiff (GB); David Powell, Cardiff (GB); and Alison Myfanwy Wakefield, Cardiff (GB)
Assigned to GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD, Sheffield (GB)
Appl. No. 15/030,994
Filed by Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
PCT Filed Oct. 8, 2014, PCT No. PCT/EP2014/071581
§ 371(c)(1), (2) Date Apr. 21, 2016,
PCT Pub. No. WO2015/058958, PCT Pub. Date Apr. 30, 2015.
Claims priority of application No. 1318814 (GB), filed on Oct. 24, 2013.
Prior Publication US 2016/0303562 A1, Oct. 20, 2016
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/52 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/12 (2013.01); B01L 2300/126 (2013.01); B01L 2300/16 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A reagent and biological sample supply apparatus for supplying a microfluidic device, the reagent and biological sample supply apparatus comprising:
a solid support formed from a material being solid and generally non-soluble, the solid support having a surface impregnated with chemicals, the chemicals including a weak base, a chelating agent, an anionic surfactant, or a chaotropic agent;
a plurality of individual aliquots being regularly spaced apart of at least one reagent being generally dry and comprising oligonucleotide primers stored on the surface of the solid support at a reagent location or locations; and
a peel-off film applied to both sides of the support;
the solid support further being suitable for storing a biological sample in a dry state at a sample location or locations on a same surface of the solid support as the reagent location or locations, spaced from the reagent location or locations,
wherein said material has a porosity which allows:
a first liquid flow through the material from the reagent location or locations on the surface on which the reagent is stored to an opposite side and which is configured to filter liquid containing dissolved or suspended reagent from at least one individual aliquot of the plurality of individual aliquots in response to the first liquid flow through the material of the solid support, and
a second liquid flow, spaced from the first liquid flow, through the material of the solid support from the sample location or locations on the surface on which the biological sample is stored to the opposite side and which is configured to filter liquid containing an extract dissolved or suspended from the stored biological sample in response to the second liquid flow through the material of the solid support, and
wherein said at least one generally dry reagent is suitable for performing an assay on biological sample recovered from the solid support by contact of the reagent from the first liquid flow with the extract from the second liquid flow.