US 12,296,069 B2
Tissue construct, methods of producing and using the same
Mark Skylar-Scott, Brookline, MA (US); Sebastien Uzel, Cambridge, MA (US); and Jennifer Lewis, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Appl. No. 16/649,056
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed Sep. 20, 2018, PCT No. PCT/US2018/051901
§ 371(c)(1), (2) Date Mar. 19, 2020,
PCT Pub. No. WO2019/060518, PCT Pub. Date Mar. 28, 2019.
Claims priority of provisional application 62/561,477, filed on Sep. 21, 2017.
Prior Publication US 2020/0289709 A1, Sep. 17, 2020
Int. Cl. A61L 27/38 (2006.01); A61B 5/00 (2006.01); A61L 27/22 (2006.01); A61L 27/36 (2006.01); A61L 27/44 (2006.01); A61L 27/50 (2006.01); A61L 27/54 (2006.01); B33Y 10/00 (2015.01); B33Y 80/00 (2015.01); C12N 5/071 (2010.01)
CPC A61L 27/3895 (2013.01) [A61L 27/222 (2013.01); A61L 27/227 (2013.01); A61L 27/3633 (2013.01); A61L 27/3808 (2013.01); A61L 27/3826 (2013.01); A61L 27/3891 (2013.01); A61L 27/44 (2013.01); A61L 27/507 (2013.01); A61L 27/54 (2013.01); B33Y 10/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0697 (2013.01); A61B 5/686 (2013.01); A61L 2430/02 (2013.01); A61L 2430/04 (2013.01); A61L 2430/10 (2013.01); A61L 2430/14 (2013.01); A61L 2430/16 (2013.01); A61L 2430/20 (2013.01); A61L 2430/22 (2013.01); A61L 2430/26 (2013.01); A61L 2430/28 (2013.01); A61L 2430/30 (2013.01); A61L 2430/32 (2013.01); A61L 2430/40 (2013.01); C12N 2502/28 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01); C12N 2533/54 (2013.01); C12N 2533/56 (2013.01); C12N 2533/72 (2013.01); C12N 2533/74 (2013.01); C12N 2533/76 (2013.01); C12N 2533/78 (2013.01); C12N 2533/80 (2013.01)] 36 Claims
 
1. A method of producing a tissue construct, comprising:
compacting embryoid bodies, organoids, cells, cell spheroids, multicellular tissue aggregates, or a combination thereof to form a granular tissue comprising a compacted assembly of embryoid bodies, organoids, cells, cell spheroids, multicellular tissue aggregates, or a combination thereof;
providing the granular tissue;
depositing one or more filaments on or in the granular tissue, each filament comprising an ink; and
gelling or fusing the granular tissue, thereby producing the tissue construct.