	US 12,296,067 B2
	Silk-hyaluronic acid tissue fillers and methods of making and using the same
Gregory H. Altman, Providence, RI (US); Rebecca L. Lacouture, Needham, MA (US); Peng Xu, Lexington, MA (US); Erlei Jin, Winchester, MA (US); Carlos J. Bosques, Arlington, MA (US); Patrick Yacono, Medford, MA (US); and Patrik Johansson, Medford, MA (US)
Assigned to EVOLVED BY NATURE, INC., Needham, MA (US)
Appl. No. 17/414,752
Filed by Evolved by Nature, Inc., Medford, MA (US)
PCT Filed Dec. 19, 2019, PCT No. PCT/US2019/067617 § 371(c)(1), (2) Date Jun. 16, 2021, PCT Pub. No. WO2020/132331, PCT Pub. Date Jun. 25, 2020.
Claims priority of provisional application 62/781,952, filed on Dec. 19, 2018.
Claims priority of provisional application 62/865,633, filed on Jun. 24, 2019.
Prior Publication US 2023/0114250 A1, Apr. 13, 2023
Int. Cl. A61L 27/26 (2006.01); A61K 8/64 (2006.01); A61K 8/73 (2006.01); A61K 8/86 (2006.01); A61L 27/36 (2006.01); A61L 27/52 (2006.01); A61L 27/54 (2006.01); A61L 27/58 (2006.01); A61Q 19/08 (2006.01)

CPC A61L 27/26 (2013.01) [A61K 8/64 (2013.01); A61K 8/735 (2013.01); A61K 8/86 (2013.01); A61L 27/3604 (2013.01); A61L 27/52 (2013.01); A61L 27/54 (2013.01); A61L 27/58 (2013.01); A61Q 19/08 (2013.01); A61K 2800/91 (2013.01); A61L 2300/402 (2013.01); A61L 2400/06 (2013.01); A61L 2430/34 (2013.01)]

20 Claims

1. A biocompatible tissue filler comprising silk fibroin fragments having an average weight average molecular weight selected from between about 1 kDa and about 250 kDa, and hyaluronic acid (HA),

wherein a portion of the HA is modified or crosslinked by one or more linker moieties comprising one or more of polyethylene glycol (PEG), polypropylene glycol (PPG), and a secondary alcohol, and

wherein modification or cross-linking is obtained using as cross-linker selected from a monoepoxy-PEG, a diepoxy-PEG, a monoglycidyl-PEG, a diglycidyl-PEG, a polyglycidyl-PEG, a monoepoxy-PPG, a diepoxy-PPG, a monoglycidyl-PPG, a diglycidyl-PPG, a polyglycidyl-PPG, and any combinations thereof.