	US 12,296,037 B2
	Fusion proteins for hydroxylating amino acids and products
Lixin Dai, Livingston, NJ (US); and Poonam Srivastava, Union City, NJ (US)
Assigned to HTL BIOTECHNOLOGY INNOVATION, INC., Nutley, NJ (US)
Appl. No. 17/269,123
Filed by HTL Biotechnology Innovation Inc., Nutley, NJ (US)
PCT Filed Aug. 16, 2019, PCT No. PCT/US2019/046883 § 371(c)(1), (2) Date Feb. 17, 2021, PCT Pub. No. WO2020/037243, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/719,419, filed on Aug. 17, 2018.
Prior Publication US 2021/0308031 A1, Oct. 7, 2021
Int. Cl. A61K 8/66 (2006.01); A61K 8/65 (2006.01); A61K 8/9728 (2017.01); A61Q 19/00 (2006.01); C12N 1/16 (2006.01); C12N 9/02 (2006.01); C12R 1/84 (2006.01)

CPC A61K 8/66 (2013.01) [A61K 8/65 (2013.01); A61K 8/9728 (2017.08); A61Q 19/00 (2013.01); C12N 1/165 (2021.05); C12N 9/0071 (2013.01); C07K 2319/00 (2013.01); C12R 2001/84 (2021.05); C12Y 114/11002 (2013.01)]

3 Claims

1. A fusion protein comprising:

a prolyl 4-hydroxylase alpha subunit; and

a prolyl 4-hydroxylase beta subunit,

wherein the prolyl 4-hydroxylase alpha subunit is at the N-terminus of the fusion protein and the prolyl 4-hydroxylase beta subunit is at the C-terminus of the fusion protein, wherein the prolyl 4-hydroxylase alpha subunit is encoded by a polynucleotide comprising SEQ ID NO: 1.