	US 12,296,001 B2
	DNA vaccine against crimean-congo hemorrhagic fever virus (CCHFV)
Kar Muthumani, Cherry Hill, NJ (US); and David Weiner, Merion, PA (US)
Assigned to The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US)
Appl. No. 17/289,297
Filed by THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY, Philadelphia, PA (US)
PCT Filed Nov. 1, 2019, PCT No. PCT/US2019/059360 § 371(c)(1), (2) Date Apr. 28, 2021, PCT Pub. No. WO2020/092880, PCT Pub. Date May 7, 2020.
Claims priority of provisional application 62/754,821, filed on Nov. 2, 2018.
Prior Publication US 2021/0401965 A1, Dec. 30, 2021
Int. Cl. A61K 39/12 (2006.01); A61K 9/00 (2006.01); A61K 38/20 (2006.01); A61K 39/00 (2006.01); A61K 39/39 (2006.01); A61P 31/14 (2006.01); C12N 7/00 (2006.01)

CPC A61K 39/12 (2013.01) [A61K 9/0009 (2013.01); A61K 38/20 (2013.01); A61K 38/208 (2013.01); A61K 38/2086 (2013.01); A61K 39/39 (2013.01); A61P 31/14 (2018.01); C12N 7/00 (2013.01); A61K 2039/53 (2013.01); A61K 2039/54 (2013.01); A61K 2039/55527 (2013.01); A61K 2039/55538 (2013.01); C12N 2760/12234 (2013.01); C12N 2760/12271 (2013.01)]

14 Claims

1. A composition comprising: a nucleic acid molecule comprising a nucleotide sequence that encodes a consensus Crimean-Congo hemorrhagic fever virus (CCHFV) antigen wherein the antigen comprises the amino acid sequence selected from the group consisting of:

a) SEQ ID NO:2, a fragment of SEQ ID NO:2 that comprises 600 or more consecutive amino acids; SEQ ID NO:2 linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 600 or more consecutive amino acids linked to an IgE signal peptide; and

b) SEQ ID NO:4, a fragment of SEQ ID NO:4 that comprises 600 or more consecutive amino acids, a fragment of SEQ ID NO:4 that comprises 600 or more consecutive amino acids linked to an IgE signal peptide.