US 12,295,974 B2
Compositions and methods for transplantation of colon microbiota
Michael J. Sadowsky, Roseville, MN (US); Alexander Khoruts, Golden Valley, MN (US); Alexa R. Weingarden, St. Paul, MN (US); and Matthew J. Hamilton, Burnsville, MN (US)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US)
Filed by Regents of the University of Minnesota, Minneapolis, MN (US)
Filed on Dec. 21, 2021, as Appl. No. 17/558,094.
Application 17/558,094 is a continuation of application No. 15/655,393, filed on Jul. 20, 2017, abandoned.
Application 15/655,393 is a continuation of application No. 15/626,898, filed on Jun. 19, 2017, abandoned.
Application 15/655,393 is a continuation of application No. 15/626,880, filed on Jun. 19, 2017, granted, now 10,251,914, issued on Apr. 9, 2019.
Application 15/626,898 is a continuation of application No. 15/594,087, filed on May 12, 2017, granted, now 10,286,011, issued on May 14, 2019.
Application 15/626,880 is a continuation of application No. 15/594,087, filed on May 12, 2017, granted, now 10,286,011, issued on May 14, 2019.
Application 15/626,880 is a continuation of application No. 15/594,319, filed on May 12, 2017, granted, now 10,286,012, issued on May 14, 2019.
Application 15/626,898 is a continuation of application No. 15/594,319, filed on May 12, 2017, granted, now 10,286,012, issued on May 14, 2019.
Application 15/594,319 is a continuation of application No. 15/261,319, filed on Sep. 9, 2016, granted, now 9,649,343, issued on May 16, 2017.
Application 15/594,087 is a continuation of application No. 15/261,319, filed on Sep. 9, 2016, granted, now 9,649,343, issued on May 16, 2017.
Application 15/594,087 is a continuation of application No. 15/173,134, filed on Jun. 3, 2016, granted, now 10,028,980, issued on Jul. 24, 2018.
Application 15/594,319 is a continuation of application No. 15/173,134, filed on Jun. 3, 2016, granted, now 10,028,980, issued on Jul. 24, 2018.
Application 15/261,319 is a continuation of application No. 14/003,411, granted, now 9,968,638, issued on May 15, 2018, previously published as PCT/US2012/028484, filed on Mar. 9, 2012.
Application 15/173,134 is a continuation of application No. 14/003,411, granted, now 9,968,638, issued on May 15, 2018, previously published as PCT/US2012/028484, filed on Mar. 9, 2012.
Claims priority of provisional application 61/450,838, filed on Mar. 9, 2011.
Prior Publication US 2022/0110981 A1, Apr. 14, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/37 (2015.01); A61K 9/00 (2006.01); A61K 9/16 (2006.01); A61K 35/00 (2006.01); A61K 35/24 (2015.01); A61K 35/74 (2015.01); A61K 35/741 (2015.01); A61K 35/742 (2015.01); A61K 45/06 (2006.01); A61K 47/08 (2006.01); A61K 47/26 (2006.01)
CPC A61K 35/37 (2013.01) [A61K 9/0053 (2013.01); A61K 9/16 (2013.01); A61K 9/1617 (2013.01); A61K 9/1623 (2013.01); A61K 35/24 (2013.01); A61K 35/74 (2013.01); A61K 35/741 (2013.01); A61K 35/742 (2013.01); A61K 45/06 (2013.01); A61K 47/08 (2013.01); A61K 47/26 (2013.01); A61K 2035/11 (2013.01); Y02A 50/30 (2018.01)] 9 Claims
 
1. A method of treating or preventing a recurrent Clostridium difficile infection in a subject in need thereof, the method comprising:
receiving a pharmaceutical composition from a fecal microbiota supply bank,
wherein the fecal microbiota supply bank comprises a plurality of fecal preparations,
wherein each fecal preparation of the plurality comprises non-pathogenic fecal bacteria extracted via standardized processing from a stool of a screened human donor unrelated to the subject,
wherein the pharmaceutical composition comprises saline, a cryoprotectant, and a single fecal preparation from the plurality of fecal preparations, and wherein the non-pathogenic fecal bacteria comprise bacteria of the phylum Firmicutes; and
rectally administering the pharmaceutical composition to the subject, wherein the administering increases the relative abundance of one or more members of the phylum Firmicutes in the intestine of the subject by at least 5% compared to before the administering.