US 12,295,848 B2
Implants including modified demineralized cortical bone fibers and methods of making same
Michele Christine McAllister, Freehold, NJ (US); David Wang, Rockville, MD (US); Michelle Bubear, Newton, NJ (US); Joed Canales, Harrison, NJ (US); Amy Chang, Kaneohe, HI (US); Roman Shikhanovich, Edison, NJ (US); Mark Spilker, Plainsboro, NJ (US); and Eric Semler, Morganville, NJ (US)
Assigned to Musculoskeletal Transplant Foundation
Filed by Musculoskeletal Transplant Foundation, Edison, NJ (US)
Filed on Oct. 14, 2022, as Appl. No. 17/966,092.
Application 16/598,354 is a division of application No. 15/159,406, filed on May 19, 2016, granted, now 10,531,957, issued on Jan. 14, 2020.
Application 17/966,092 is a continuation of application No. 16/598,354, filed on Oct. 10, 2019, granted, now 11,596,517.
Claims priority of provisional application 62/331,071, filed on May 3, 2016.
Claims priority of provisional application 62/164,827, filed on May 21, 2015.
Prior Publication US 2023/0030907 A1, Feb. 2, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61F 2/28 (2006.01); A61L 27/00 (2006.01); A61L 27/36 (2006.01)
CPC A61F 2/28 (2013.01) [A61L 27/3604 (2013.01); A61L 27/3608 (2013.01); A61L 27/365 (2013.01); A61L 27/3683 (2013.01); A61F 2240/001 (2013.01); A61L 2400/18 (2013.01); A61L 2430/02 (2013.01); A61L 2430/38 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for producing an implant from modified demineralized cortical bone fibers which is at least partially opaque, said method comprising the steps of:
contacting the demineralized cortical bone fibers with a salt solution prior to lyophilizing;
shaping a mass of the demineralized cortical bone fibers to form an implant having a desired shape;
forming one or more at least partially radio opaque portions of the implant, after the shaping step and before the lyophilizing step, by
at least partially remineralizing one or more regions of the implant by alternately contacting implant with an aqueous calcium chloride solution and an aqueous sodium phosphate solution, with agitation, to form one or more remineralized regions each of which is at least partially opaque,
and
lyophilizing the implant,
to produce a lyophilized implant that is at least partially radio opaque and has a residual moisture content of less than about 6% by weight, based on the total weight of the implant, and a complete rehydration time of less than about 30 minutes, and wherein said lyophilized implant remains cohesive and retains its shape upon complete hydration.