US 12,293,519 B2
Cell evaluation method, cell evaluation system and program
Hideki Taniguchi, Yokohama (JP); Takanori Takebe, Yokohama (JP); Momotaro Ishikawa, Kamakura (JP); Masafumi Yamashita, Fujisawa (JP); and Yasujiro Kiyota, Tokyo (JP)
Assigned to PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY, Kanagawa (JP); and NIKON CORPORATION, Tokyo (JP)
Appl. No. 17/770,977
Filed by PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY, Kanagawa (JP); and NIKON CORPORATION, Tokyo (JP)
PCT Filed Nov. 2, 2020, PCT No. PCT/JP2020/041084
§ 371(c)(1), (2) Date Aug. 24, 2022,
PCT Pub. No. WO2021/085649, PCT Pub. Date May 6, 2021.
Claims priority of application No. 2019-198409 (JP), filed on Oct. 31, 2019.
Prior Publication US 2023/0154003 A1, May 18, 2023
Int. Cl. G06T 7/00 (2017.01); C12M 1/34 (2006.01)
CPC G06T 7/0016 (2013.01) [C12M 41/46 (2013.01); G06T 2207/30004 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A cell evaluation method for evaluating cell differentiation in a culture process including a cell differentiation-inducing process of stem cells in an undifferentiated state into mature somatic cells, the cell evaluation method comprising:
acquiring a first evaluation index which is a feature acquired from an image in which comparative target cells in the culture process are imaged and a first index calculated using the first evaluation index;
calculating, on the basis of the first evaluation index, a second index which is a feature acquired from an image in which evaluation target cells different from the comparative target cells are imaged; and
evaluating differentiation of the evaluation target cells by comparing the first index with the second index on the basis of a correlation between a value of an area of a muscle region of the stem cells in an undifferentiated state and an amount of the substance produced by the mature somatic cells, wherein
the first index includes the value of an area of a muscle region in a process of maintaining and culturing the cells in an undifferentiated state.