US 12,293,517 B2
Systems for cell shape estimation
Joerg Bredno, Tucson, AZ (US); Konstanty Korski, Penzberg (DE); and Oliver Grimm, Penzberg (DE)
Assigned to VENTANA MEDICAL SYSTEMS, INC., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Oct. 30, 2023, as Appl. No. 18/497,974.
Application 18/497,974 is a continuation of application No. 16/949,030, filed on Oct. 9, 2020, granted, now 11,842,483.
Application 16/949,030 is a continuation of application No. PCT/EP2019/059181, filed on Apr. 11, 2019.
Claims priority of provisional application 62/657,509, filed on Apr. 13, 2018.
Prior Publication US 2024/0078670 A1, Mar. 7, 2024
Int. Cl. G06T 7/00 (2017.01); G06V 20/69 (2022.01)
CPC G06T 7/0012 (2013.01) [G06V 20/695 (2022.01); G06T 2207/10004 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30242 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A computer-implemented method comprising:
accessing an image of a stained biological sample;
processing the image to generate predictive cell motility labels for the image, wherein the image is processed by:
detecting, using a trained classifier, lymphocytes in the image;
identifying outlines of the lymphocytes by applying image enhancing filters to the image, wherein applying the image enhancing filters to the image causes one or more regions of the image to be discarded and one or more regions of the image to be retained;
computing shape metrics for the lymphocytes based on the outlines, wherein a shape metric of the shape metrics is computed for each lymphocyte of the lymphocytes;
for each respective lymphocyte of the lymphocytes, assigning a respective predictive cell motility label of a plurality of predictive cell motility labels to the respective lymphocyte based on a respective shape metric of the shape metrics, the respective shape metric indicative of a shape of the respective lymphocyte, wherein the respective predictive cell motility label corresponds to a letter and/or number that identifies a likelihood that the respective lymphocyte is motile; and
generating an overlay for the image using the predictive cell motility labels, wherein the overlay provides a visualization of the lymphocytes and predicted motilities of the lymphocytes.