US 12,292,446 B2
Kits for analysis using nucleic acid encoding and/or label
Mark S. Chee, Encinitas, CA (US); John M. Beierle, San Diego, CA (US); Norihito Muranaka, San Diego, CA (US); Kevin L. Gunderson, Encinitas, CA (US); Michael Phillip Weiner, San Diego, CA (US); Lei Shi, San Diego, CA (US); Robert C. James, San Diego, CA (US); and Luca Monfregola, San Diego, CA (US)
Assigned to ENCODIA, INC., San Diego, CA (US)
Filed by Encodia, Inc., San Diego, CA (US)
Filed on Jul. 29, 2024, as Appl. No. 18/787,946.
Application 18/787,946 is a continuation of application No. 17/819,263, filed on Aug. 11, 2022, granted, now 12,130,291.
Application 17/819,263 is a continuation of application No. 16/760,028, granted, now 11,513,126, issued on Nov. 29, 2022, previously published as PCT/US2018/058565, filed on Oct. 31, 2018.
Claims priority of provisional application 62/583,448, filed on Nov. 8, 2017.
Claims priority of provisional application 62/582,312, filed on Nov. 6, 2017.
Claims priority of provisional application 62/579,844, filed on Oct. 31, 2017.
Prior Publication US 2024/0377409 A1, Nov. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C40B 40/10 (2006.01); C40B 70/00 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/6842 (2013.01) [C40B 40/10 (2013.01); C40B 70/00 (2013.01); G01N 33/6845 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A kit for analyzing a polypeptide, comprising:
(a) a functionalizing reagent that reacts with an N-terminal amino acid (NTAA) of the polypeptide to generate a functionalized NTAA of the polypeptide;
(b) one or more nucleic acid recording tags configured to associate directly or indirectly with the polypeptide or with a component of the polypeptide obtained by a cleavage of the functionalized NTAA of the polypeptide, wherein each of the one or more nucleic acid recording tags comprises a unique molecular identifier (UMI); and
(c) one or more binding agents each comprising:
(i) a binding moiety that binds to the functionalized NTAA of the polypeptide or the component of the polypeptide obtained by the cleavage of the functionalized NTAA of the polypeptide; and
(ii) a nucleic acid coding tag attached to the binding moiety and comprising a barcode sequence that comprises identifying information regarding the binding moiety;
wherein the one or more nucleic acid recording tags and the one or more nucleic acid coding tags are configured to allow transfer of sequence information of the barcode sequence and/or the UMI by ligation or primer extension, upon binding between each binding agent and the functionalized NTAA of the polypeptide or the component of the polypeptide, thereby generating one or more extended nucleic acid constructs each comprising the barcode sequence or a complement thereof and the UMI or a complement thereof.