US 12,292,444 B2
Methods and compositions related to modified green fluorescent protein (GFP) with an embedded foreign peptide
Vladimir N. Uversky, Tampa, FL (US); and Bogdan S. Melnik, Pushchino (RU)
Assigned to University of South Florida, Tampa, FL (US)
Filed by University of South Florida, Tampa, FL (US)
Filed on Mar. 31, 2021, as Appl. No. 17/219,119.
Claims priority of provisional application 63/003,572, filed on Apr. 1, 2020.
Prior Publication US 2021/0311068 A1, Oct. 7, 2021
Int. Cl. G01N 33/58 (2006.01); G01N 33/50 (2006.01); G01N 33/542 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/582 (2013.01) [G01N 33/5041 (2013.01); G01N 33/542 (2013.01); G01N 33/6842 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A composition comprising a modified green fluorescent protein (GFP) molecule and a bactenecin, wherein the bactenecin consists of SEQ ID NO: 1 and replaces at least a part of a central α-helix region of a GFP molecule comprising SEQ ID NO: 2, and wherein the modified GFP molecule comprises the amino acid substitutions F8D, I167D, and Q177E, which confer stability to the composition.