US 12,292,440 B2
Sandwich assays in droplets
Michael L. Samuels, Windham, NH (US); and Darren Roy Link, Lexington, MA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on May 2, 2024, as Appl. No. 18/653,506.
Application 18/653,506 is a continuation of application No. 18/305,650, filed on Apr. 24, 2023.
Application 18/305,650 is a continuation of application No. 16/937,908, filed on Jul. 24, 2020, granted, now 11,635,427, issued on Apr. 25, 2023.
Application 16/937,908 is a continuation of application No. 15/415,276, filed on Jan. 25, 2017, granted, now 10,761,090, issued on Sep. 1, 2020.
Application 15/415,276 is a continuation of application No. 13/250,702, filed on Sep. 30, 2011, granted, now 9,562,897, issued on Feb. 7, 2017.
Claims priority of provisional application 61/388,413, filed on Sep. 30, 2010.
Prior Publication US 2024/0280569 A1, Aug. 22, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); B01F 33/3011 (2022.01); B01F 33/302 (2022.01); C12Q 1/6874 (2018.01); C40B 30/04 (2006.01); C40B 40/00 (2006.01); C40B 40/04 (2006.01); C40B 70/00 (2006.01); G01N 33/532 (2006.01)
CPC G01N 33/54366 (2013.01) [C12Q 1/6874 (2013.01); C40B 40/00 (2013.01); C40B 40/04 (2013.01); C40B 70/00 (2013.01); G01N 33/532 (2013.01); B01F 33/3011 (2022.01); B01F 33/3021 (2022.01); C40B 30/04 (2013.01)] 17 Claims
 
1. A method comprising:
incubating a target analyte with binding agents, wherein the binding agents comprise binders linked to oligonucleotides,
wherein each binding agent is provided with barcode sequences that include a target analyte identifier and a unique identifier unique to the binding agent;
binding one of the binding agents to the target analyte; and
sequencing the barcode sequences to detect the target analyte.