	US 12,292,439 B2
	Method for manufacturing surface-enhanced Raman scattering-based substrate for detecting target substance, substrate manufactured thereby for detecting target substance, and method for detecting target substance by using same substrate
Yeon Ho Choi, Seoul (KR); and Hyun Ku Shin, Seoul (KR)
Assigned to Exopert Corporation, Seoul (KR)
Appl. No. 17/311,720
Filed by Exopert Corporation, Seoul (KR)
PCT Filed Mar. 27, 2020, PCT No. PCT/KR2020/004161 § 371(c)(1), (2) Date Jun. 8, 2021, PCT Pub. No. WO2020/197305, PCT Pub. Date Oct. 1, 2020.
Claims priority of application No. 10-2019-0035295 (KR), filed on Mar. 27, 2019.
Prior Publication US 2022/0026423 A1, Jan. 27, 2022
Int. Cl. G01N 1/38 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01); G01N 21/65 (2006.01); G01N 33/543 (2006.01); G01N 33/553 (2006.01)

CPC G01N 33/54346 (2013.01) [G01N 1/38 (2013.01); G01N 21/658 (2013.01); G01N 33/553 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); G01N 2001/386 (2013.01)]

10 Claims

1. A method for manufacturing a surface-enhanced Raman scattering (SERS)-based substrate for detecting a target substance, comprising:

a step of forming a primary capture structure with a target substance and a bioreceptor bound by immersing a bioreceptor-attached substrate in a sample solution comprising a target substance;

a step of disposing the substrate with the primary capture structure formed in a centrifuge tube and adding a solution comprising metal nanoparticles into the centrifuge tube; and

a step of forming a secondary capture structure directly coating the metal nanoparticles on a surface of the target substance by operating the centrifuge tube.