US 12,292,437 B2
Fluorescence histo-tomography (FHT) systems and methods
John W. Hoppin, Boston, MA (US); John V. Frangioni, Wayland, MA (US); Robert William Holt, Boston, MA (US); Mohammed Q. Qutaish, Medford, MA (US); Marc Edward Seaman, Norwood, MA (US); Mark W. Bordo, Worcester, MA (US); and Jacob Yost Hesterman, Newton, MA (US)
Assigned to Emit Imaging, Inc., Boston, MA (US)
Filed by Emit Imaging, Inc., Baltimore, MD (US)
Filed on Jul. 11, 2022, as Appl. No. 17/861,730.
Application 17/861,730 is a continuation of application No. 16/239,634, filed on Jan. 4, 2019, granted, now 11,385,222.
Application 16/239,634 is a continuation of application No. 15/253,149, filed on Aug. 31, 2016, granted, now 10,209,242, issued on Feb. 19, 2019.
Application 15/253,149 is a continuation in part of application No. 15/158,928, filed on May 19, 2016, granted, now 9,799,113, issued on Oct. 24, 2017.
Claims priority of provisional application 62/211,930, filed on Aug. 31, 2015.
Claims priority of provisional application 62/164,800, filed on May 21, 2015.
Prior Publication US 2022/0341916 A1, Oct. 27, 2022
Int. Cl. A61B 5/00 (2006.01); G01N 33/50 (2006.01); G06T 5/73 (2024.01); G06T 7/30 (2017.01); H04N 23/51 (2023.01); H04N 23/90 (2023.01)
CPC G01N 33/5023 (2013.01) [A61B 5/0073 (2013.01); G06T 5/73 (2024.01); G06T 7/30 (2017.01); H04N 23/51 (2023.01); H04N 23/90 (2023.01); A61B 5/0071 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of performing fluorescence histo-tomography (FHT) comprising:
capturing, by an imaging device mounted with respect to a chamber of a slicing apparatus, a first image of a first block face of a specimen retained within the chamber, the first image captured in a visible spectrum;
capturing, by the imaging device, a second image of the first block face under white light and fluorescence excitation illumination;
positioning the imaging device away from the chamber;
operating the slicing apparatus to expose a second block face of the specimen;
repositioning the imaging device with respect to the chamber; and
capturing a third image of the second block face.