US 12,292,426 B1
Method for identification of characteristic components in Kai-Xin-San by thin-layer chromatography (TLC)
Caiyun Zhang, Hefei (CN); Wenwen Tao, Hefei (CN); Xiaoxiao Shan, Hefei (CN); Dawei Li, Hefei (CN); Lele Zhou, Hefei (CN); Wenkang Tao, Hefei (CN); and Hongyan Cheng, Hefei (CN)
Assigned to Anhui University of Chinese Medicine, Hefei (CN)
Filed by Anhui University of Chinese Medicine, Hefei (CN)
Filed on Jan. 7, 2025, as Appl. No. 19/012,222.
Claims priority of application No. 202411120819.1 (CN), filed on Aug. 15, 2024.
Int. Cl. G01N 30/94 (2006.01); G01N 33/15 (2006.01)
CPC G01N 30/94 (2013.01) [G01N 33/15 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for identification of characteristic components in a Kai-Xin-San by thin-layer chromatography (TLC), comprising the following steps:
collecting a first test solution of Kai-Xin-San, a first negative test solution of Kai-Xin-San, a first reference medicinal material solution, and a first standard solution, then spotting on a first silica gel G thin-layer plate to determine characteristic components in ginseng radix et rhizoma and polygalae radix, respectively;
collecting a second test solution of Kai-Xin-San, a second negative test solution of Kai-Xin-San, a second reference medicinal material solution, and a second standard solution, then spotting on a second silica gel G thin-layer plate to determine characteristic components in Poria and Acori tatarinowii Rhizoma, respectively;
developing the first silica gel G thin-layer plate with a developing solvent of dichloromethane (DCM)-n-butanol (n-BuOH)-water, developing the second silica gel G thin-layer plate with a developing solvent of DCM-ethyl acetate-formic acid, then taking out the first silica gel G thin-layer plate from the developing solvent and the second silica gel G thin-layer plate from the developing solvent to allow air-drying after the developing is completed; wherein DCM, n-BuOH, and water in the developing solvent of DCM-n-BuOH-water are at a volume ratio of 7-8:4:1, and DCM, ethyl acetate, and formic acid in the developing solvent of DCM-ethyl acetate-formic acid are at a volume ratio of 12:3:0.25-0.5; and
spraying the first air-dried silica gel G thin-layer plate with a sulfuric acid-ethanol solution for color development inspection, and spraying the second air-dried silica gel G thin-layer plate with a vanillin sulfuric acid-ethanol mixed solution for color development inspection.