US 12,292,424 B2
Two-dimensional LC-MS/MS systems
Wei-Lien Chuang, Bridgewater, NJ (US); Felipe Gazos Lopes, Bridgewater, NJ (US); Joshua Pacheco, Bridgewater, NJ (US); and Gerard Sanderink, Bridgewater, NJ (US)
Assigned to Genzyme Corporation, Cambridge, MA (US)
Appl. No. 17/615,291
Filed by Genzyme Corporation, Cambridge, MA (US)
PCT Filed May 29, 2020, PCT No. PCT/US2020/035231
§ 371(c)(1), (2) Date Nov. 30, 2021,
PCT Pub. No. WO2020/243502, PCT Pub. Date Dec. 3, 2020.
Claims priority of provisional application 62/855,636, filed on May 31, 2019.
Prior Publication US 2022/0252559 A1, Aug. 11, 2022
Int. Cl. G01N 30/46 (2006.01); B01D 15/18 (2006.01); B01D 15/32 (2006.01); G01N 30/02 (2006.01); G01N 30/14 (2006.01); G01N 30/72 (2006.01)
CPC G01N 30/468 (2013.01) [B01D 15/1878 (2013.01); B01D 15/322 (2013.01); B01D 15/325 (2013.01); G01N 30/14 (2013.01); G01N 30/463 (2013.01); G01N 30/7233 (2013.01); G01N 2030/027 (2013.01); G01N 2405/08 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for analyzing one or more analytes in a source sample, comprising the steps of:
(a) extracting the one or more analytes from the source sample with an extraction solvent to obtain an extraction sample;
(b) applying the extraction sample and a solvent system to a first liquid chromatography (LC) column with an LC pump system, wherein the first LC column is directly connected to a second LC column through a tube with a diverting valve;
(c) setting the diverting valve to a first position at a first predetermined time such that the solvent effluent from the first LC column is directed to waste;
(d) setting the diverting valve to a second position at a second predetermined time such that the solvent effluent from the first LC column enters the second LC column for further separation;
(e) repeating steps (c) and (d) as needed; and
(f) analyzing the chromatographically separated sample obtained from the second LC column,
wherein the method utilizes only one LC pump system for both LC columns, and
wherein the extraction solvent is compatible with the solvent system.