US 12,292,381 B2
Fluorescence observation apparatus, fluorescence observation system, and fluorescence observation method
Ayumu Taguchi, Tokyo (JP)
Assigned to SONY GROUP CORPORATION, Tokyo (JP)
Appl. No. 17/996,353
Filed by SONY GROUP CORPORATION, Tokyo (JP)
PCT Filed Apr. 7, 2021, PCT No. PCT/JP2021/014734
§ 371(c)(1), (2) Date Oct. 17, 2022,
PCT Pub. No. WO2021/215248, PCT Pub. Date Oct. 28, 2021.
Claims priority of application No. 2020-077859 (JP), filed on Apr. 25, 2020.
Prior Publication US 2023/0213450 A1, Jul. 6, 2023
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6486 (2013.01) [G01N 21/6408 (2013.01); G01N 2021/641 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A fluorescence observation apparatus, comprising:
an irradiation unit configured to apply a plurality of kinds of excitation light of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample that is labeled with a composite phosphor, wherein the composite phosphor includes two or more kinds of fluorescent molecules at a specific composition ratio;
a detection unit configured to detect fluorescence generated at each of the plurality of spatially or temporally different positions, wherein the fluorescence is generated based on the application of the plurality of kinds of excitation light by the irradiation unit; and
a calculation unit configured to determine a distribution of pieces of the composite phosphor based on a fluorescence signal of each piece of the detected fluorescence, wherein
the fluorescence signal is obtained based on a detection result of the detection unit, and
the fluorescence signal shows a fluorescence intensity corresponding to a position of the plurality of spatially or temporally different positions in the biological sample.