US 12,292,046 B2
Microfluidic filter device and method for dissociation of tissue and cell aggregates and enrichment of single cells
Jered Haun, Irvine, CA (US); Xiaolong Qiu, Irvine, CA (US); Marissa Noelani Pennell, Rocklin, CA (US); and Elliott E. Hui, South Pasadena, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Appl. No. 17/058,634
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
PCT Filed May 29, 2019, PCT No. PCT/US2019/034470
§ 371(c)(1), (2) Date Nov. 24, 2020,
PCT Pub. No. WO2019/232100, PCT Pub. Date Dec. 5, 2019.
Claims priority of provisional application 62/678,171, filed on May 30, 2018.
Prior Publication US 2021/0205810 A1, Jul. 8, 2021
Int. Cl. B01D 63/00 (2006.01); B01D 63/08 (2006.01); B01L 3/00 (2006.01); F04B 43/04 (2006.01)
CPC F04B 43/04 (2013.01) [B01D 63/005 (2013.01); B01D 63/088 (2013.01); B01L 3/502761 (2013.01); B01D 2319/025 (2013.01); B01D 2325/0283 (2022.08); B01L 2200/027 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A microfluidic tissue dissociation and filtration device comprising:
an inlet coupled to a first microfluidic channel at an upstream location, the first microfluidic channel coupled to a first outlet at a downstream location, wherein the first microfluidic channel is disposed in a first layer of the microfluidic tissue dissociation and filtration device;
a second microfluidic channel located within a second layer of the microfluidic tissue dissociation and filtration device;
a first filter membrane interposed between the first microfluidic channel and the second microfluidic channel, wherein the second microfluidic channel is in fluidic communication with the first microfluidic channel by a first connecting fluid passageway containing the first filter membrane;
a second outlet coupled to a downstream location of the second microfluidic channel;
a second filter membrane interposed between the second outlet and the second microfluidic channel and contained in a second connecting fluid passageway that fluidically connects the second microfluidic channel to the second outlet;
a pump connected to a source of tissue or cellular aggregates contained within a fluid, the pump further fluidically connected to the inlet; and
wherein the first filter membrane comprises pores having diameters of d1 and wherein the second filter membrane comprises pores having diameters of d2, wherein d1>d2 and wherein the first filter membrane has pore diameters that are at least two times the pore diameters of the second filter membrane, and wherein the second filter membrane is configured to allow passage of single cells and fluid and generates an increase in yield of single cells from the tissue or cell aggregates of at least 3-fold in the second outlet.