US 12,291,754 B2
Culture-related process optimization method and culture-related process optimization system
Yosuke Ozawa, Tokyo (JP); Taku Tsuzuki, Tokyo (JP); and Tatsuki Higashi, Tokyo (JP)
Assigned to EPISTRA INC., Tokyo (JP)
Appl. No. 18/688,351
Filed by EPISTRA INC., Tokyo (JP)
PCT Filed Aug. 30, 2022, PCT No. PCT/JP2022/032667
§ 371(c)(1), (2) Date Feb. 29, 2024,
PCT Pub. No. WO2023/033005, PCT Pub. Date Mar. 9, 2023.
Claims priority of application No. 2021-141911 (JP), filed on Aug. 31, 2021.
Prior Publication US 2024/0327935 A1, Oct. 3, 2024
Int. Cl. C12Q 3/00 (2006.01)
CPC C12Q 3/00 (2013.01) 14 Claims
OG exemplary drawing
 
1. A culture-related process optimization method in a culture-related process optimization system comprising a culture-related process optimization device, the culture-related process optimization method comprising:
an acquisition step of acquiring, by a computer of the culture-related process optimization device, an initial execution procedure table that serves as a starting point of a search, wherein the initial execution procedure table defines one or a plurality of operations to be performed in a culture-related process relevant to culture of cells, and defines contents related to the operations;
a variable parameter item identification step of identifying, by the computer, one or a plurality of variable parameter items for which a variable parameter value is set in the initial execution procedure table;
a search range determining step of determining, by the computer, a search range indicating a range of the variable parameter value to be set in each of the variable parameter items;
a template execution procedure generation step of generating, by the computer, a template execution procedure in which the search range is set by rewriting the initial execution procedure table;
an execution procedure generation step of selecting, by the computer, the variable parameter value in the search range and setting the selected variable parameter value for the variable parameter item identified in the variable parameter item identification step on the basis of a previous execution performance result and an evaluation performance result thereof and thereby generating an execution procedure of the culture-related process;
an execution result acquisition step of acquiring, by the computer, an execution result obtained in response to an execution subject actually executing the execution procedure in an execution environment;
an evaluation result acquisition step of acquiring, by the computer, an evaluation result with respect to the execution result, wherein the evaluation result is at least one of cost, yield, quality, and execution time related to the execution procedure, variations in the cost, the yield, the quality, and the execution time, and deviations from given target values of the cost, the yield, the quality, and the execution time;
a storage step of recording, in a storage of the culture-related process optimization device, the execution procedure, the selected variable parameter value, the execution result, and the evaluation result in association with each other in a database; and
a determination step of determining, by the computer, whether the evaluation result is a predetermined evaluation result, wherein
in the determination step,
upon determining that the evaluation result is not the predetermined evaluation result, changing the variable parameter value within the search range set in the template execution procedure to generate a new execution procedure, and
upon determining that the evaluation result is the predetermined evaluation result, terminating the culture-related process optimization method.