US 12,291,748 B2
Acridinium ester-containing compounds and methods of using the same for chemiluminescence-based one-color sequencing
Yan Chen, Sunnyvale, CA (US); and Handong Li, San Jose, CA (US)
Assigned to MGI Holdings Co., Limited, Shenzhen (CN)
Filed by MGI Holdings Co., Limited, Guangdong (CN)
Filed on Aug. 6, 2020, as Appl. No. 16/987,092.
Claims priority of provisional application 62/883,760, filed on Aug. 7, 2019.
Prior Publication US 2021/0040556 A1, Feb. 11, 2021
Int. Cl. C12Q 1/6874 (2018.01); C07D 401/12 (2006.01); G01N 21/76 (2006.01)
CPC C12Q 1/6874 (2013.01) [C07D 401/12 (2013.01); G01N 21/76 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A compound including a nucleotide moiety of the following formula:

OG Complex Work Unit Chemistry
wherein:
R1 is a linking group comprising a C1-C8 alkyl sulfonyl piperidine;
R4, R5, and R6 are each independently selected from the group consisting of hydrogen, —CH3, —OCH3, substituted or unsubstituted C1-8 alkyl, substituted or unsubstituted C2-8 alkenyl, and substituted or unsubstituted C2-8 alkynyl, wherein the substituted alkyl group or the substituted alkenyl group is independently substituted with one or more members selected from the group consisting of a hydroxyl, a halogen, and a carboxyl group;
R7 is a nucleotide moiety of the following formula:

OG Complex Work Unit Chemistry
 wherein
R8 is the nitrogenous base, which is a member selected from the group consisting of:

OG Complex Work Unit Chemistry
 and
R9 is hydrogen or a blocking group; and
X− is a counteranion.