US 12,291,747 B2
Linked target capture
Joel Pel, Vancouver (CA); and Andrea Marziali, North Vancouver (CA)
Assigned to NCAN GENOMICS, INC., Vancouver (CA)
Filed by NCAN Genomics, Inc., Vancouver (CA)
Filed on Aug. 1, 2024, as Appl. No. 18/791,679.
Application 18/791,679 is a continuation of application No. 18/443,715, filed on Feb. 16, 2024.
Application 18/443,715 is a continuation of application No. 17/178,614, filed on Feb. 18, 2021, granted, now 11,905,556, issued on Feb. 20, 2024.
Application 17/178,614 is a continuation of application No. 16/239,100, filed on Jan. 3, 2019, granted, now 10,961,573, issued on Mar. 30, 2021.
Application 16/239,100 is a continuation in part of application No. 16/088,720, filed on Sep. 26, 2018, granted, now 10,961,568, issued on Mar. 30, 2021.
Application 17/178,614 is a continuation of application No. 16/088,720, granted, now 10,961,568, issued on Mar. 30, 2021, previously published as PCT/IB2017/051779, filed on Mar. 28, 2017.
Claims priority of provisional application 62/409,633, filed on Oct. 18, 2016.
Claims priority of provisional application 62/359,468, filed on Jul. 7, 2016.
Claims priority of provisional application 62/313,974, filed on Mar. 28, 2016.
Prior Publication US 2024/0384340 A1, Nov. 21, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01)
CPC C12Q 1/6869 (2013.01) 15 Claims
 
1. A method of sequencing a nucleic acid, the method comprising:
capturing a sense strand and an antisense strand of a nucleic acid fragment in a partition;
amplifying the nucleic acid fragment in the partition to produce amplification products that include substantially identical copies made from both the sense strand and the antisense strand of the nucleic acid fragment, wherein the substantially identical copies made from both the sense strand and the antisense strand are joined with a linker in phase so as to prevent binding there between; and
sequencing the amplification products in a single sequencing reaction.