US 12,291,746 B2
Methods of sequencing linked fragments
Milenko Despotovic, Richmond (CA); Joel Pel, Vancouver (CA); and Andrea Marziali, North Vancouver (CA)
Assigned to NCAN GENOMICS, INC., Vancouver (CA)
Filed by NCAN Genomics, Inc., Vancouver (CA)
Filed on Aug. 1, 2024, as Appl. No. 18/791,671.
Application 18/791,671 is a continuation of application No. 18/489,381, filed on Oct. 18, 2023.
Application 18/489,381 is a continuation of application No. 17/060,853, filed on Oct. 1, 2020, granted, now 11,827,930, issued on Nov. 28, 2023.
Application 17/060,853 is a continuation of application No. 16/008,702, filed on Jun. 14, 2018, granted, now 10,829,813, issued on Nov. 10, 2020.
Application 16/008,702 is a continuation of application No. 14/930,227, filed on Nov. 2, 2015, granted, now 10,000,799, issued on Jun. 19, 2018.
Claims priority of provisional application 62/074,991, filed on Nov. 4, 2014.
Prior Publication US 2024/0392367 A1, Nov. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01)
CPC C12Q 1/6869 (2013.01) 10 Claims
 
1. A method for preparing a sequencing library, the method comprising:
ligating adapters onto a plurality of nucleic acid fragments;
exposing the fragments to joined primers attached to a solid support; and
extending (i) a first primer of the joined primers to make a copy of a sense strand from one fragment of the plurality of fragments and (ii) a second primer of the joined primers to make a copy of a copy of an anti-sense strand of the one fragment, wherein the copy of the sense strand and the copy of the copy of the anti-sense strand are linked copies with an identical sequence;
amplifying the linked copies with the identical sequence to generate amplicons attached to the solid support;
sequencing the amplicons; and
identifying an error introduced during the amplifying step when the sequencing step gives a signal indicating disagreement between two bases at corresponding positions in the amplicons.