US 12,291,743 B2
Method for evaluating tea plant (+)-catechin content
Kaixing Fang, Guangdong (CN); Hualing Wu, Guangdong (CN); Xiaohui Jiang, Guangdong (CN); Hongjian Li, Guangdong (CN); Qiushuang Wang, Guangdong (CN); Dandan Qin, Guangdong (CN); Chendong Pan, Guangdong (CN); and Bo Li, Guangdong (CN)
Assigned to TEA RESEARCH INSTITUTE, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES, Guangdong (CN)
Filed by TEA RESEARCH INSTITUTE, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES, Guangdong (CN)
Filed on May 13, 2024, as Appl. No. 18/662,886.
Application 18/662,886 is a division of application No. 17/254,302, abandoned, previously published as PCT/CN2019/110920, filed on Oct. 14, 2019.
Claims priority of application No. 201910833662.X (CN), filed on Sep. 4, 2019; application No. 201910833670.4 (CN), filed on Sep. 4, 2019; application No. 201910833687.X (CN), filed on Sep. 4, 2019; application No. 201910833698.8 (CN), filed on Sep. 4, 2019; and application No. 201910834177.4 (CN), filed on Sep. 4, 2019.
Prior Publication US 2024/0309433 A1, Sep. 19, 2024
Int. Cl. C12Q 1/6827 (2018.01)
CPC C12Q 1/6827 (2013.01) [C12Q 2600/156 (2013.01)] 1 Claim
 
1. A method for evaluating tea plant (+)-catechin content, comprising detecting a genotype of a molecular marker by using a pair of primers of the molecular marker for detecting and evaluating the tea plant (+)-catechin content, wherein the primers consist of nucleotide sequences shown as SEQ ID NO:17 and SEQ ID NO:18, and the molecular marker is located at a SNP site of tea genomes Scaffold3727:442660, which is the 501st base of SEQ ID NO:16,
when the genotype of a sample is double mutant AA, the (+)-catechin content in a dry matter in the tea plant is higher than that of a normal average of the sample of which the genotype is a wild type GG or a single mutant GA.