US 12,291,741 B2
Detection units and methods for detecting a target analyte
Alfredo Andres Celedon, Columbia, MD (US); Saravana Radha Krishna Murthy, Gaithersburg, MD (US); Zhiguang Xu, Baltimore, MD (US); Danielle Elise Schultz, Gaithersburg, MD (US); and Troy Allen Horn, Reisterstown, MD (US)
Assigned to Scanogen Inc., Baltimore, MD (US)
Filed by Scanogen Inc., Baltimore, MD (US)
Filed on Oct. 25, 2022, as Appl. No. 17/973,055.
Application 17/973,055 is a continuation of application No. 16/217,253, filed on Dec. 12, 2018, granted, now 11,505,818.
Application 16/217,253 is a continuation of application No. 15/033,629, granted, now 10,179,930, issued on Jan. 15, 2019, previously published as PCT/US2015/014616, filed on Feb. 5, 2015.
Claims priority of provisional application 61/936,863, filed on Feb. 6, 2014.
Prior Publication US 2023/0039492 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/6816 (2018.01); G01N 27/327 (2006.01); G01N 27/414 (2006.01); G01N 27/74 (2006.01); G01N 33/543 (2006.01)
CPC C12Q 1/6816 (2013.01) [G01N 27/3271 (2013.01); G01N 27/3276 (2013.01); G01N 27/4145 (2013.01); G01N 27/745 (2013.01); G01N 33/54306 (2013.01); G01N 33/54313 (2013.01); G01N 33/54326 (2013.01); G01N 33/54373 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A kit for detecting a target analyte in a sample, comprising:
a) a first probe comprising a first region configured to directly bind the target analyte at a first location and a second region configured to couple to a particle, wherein the first probe cannot specifically couple to a solid support in the absence of the target analyte; and
b) a second probe comprising a first region configured to directly bind the target analyte at a second location different than the first location, and a second region configured to couple to the solid support, wherein the second probe cannot specifically couple to the particle in the absence of the target analyte;
wherein the first probe and the second probe can bind to the target analyte, if present in the sample, to form a complex such that the particle can be indirectly coupled to the solid support, and wherein the length of the complex from where the first probe couples to the particle to where the second probe couples to the solid support is about 1.0 micron to about 20 microns.
 
11. A method of detecting a target analyte in a sample comprising:
a) providing a detectable particle indirectly coupled to a solid support via a complex if the target analyte is present in the sample, the complex formed from:
i) a first probe comprising a first region configured to directly bind the target analyte at a first location and a second region configured to couple to the detectable particle, wherein the first probe cannot specifically couple to the solid support in the absence of the target analyte; and
ii) a second probe comprising a first region configured to directly bind the target analyte at a second location different than the first location, and a second region configured to couple to the solid support, wherein the second probe cannot specifically couple to the detectable particle in the absence of the target analyte;
iii) the target analyte,
wherein at least one of the first probe, the second probe, and a region of the target analyte between the first location and the second location comprises an elongated region of about 1.0 micron to about 20 microns long;
b) either i) applying a force to the complex or ii) measuring the Brownian motion of the detectable particle.