US 12,291,739 B2
Compositions and methods for enriching populations of nucleic acids
Timothy A. Blauwkamp, Palo Alto, CA (US); Fred Christians, Los Altos Hills, CA (US); Igor D. Vilfan, East Palo Alto, CA (US); Scott Smith, San Francisco, CA (US); and Michael Kertesz, Menlo Park, CA (US)
Assigned to Karius, Inc., Redwood City, CA (US)
Filed by Karius, Inc., Redwood City, CA (US)
Filed on Jul. 30, 2021, as Appl. No. 17/390,550.
Application 17/390,550 is a continuation of application No. 15/157,374, filed on May 17, 2016, granted, now 11,111,520.
Claims priority of provisional application 62/334,348, filed on May 10, 2016.
Claims priority of provisional application 62/163,273, filed on May 18, 2015.
Prior Publication US 2022/0154246 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6888 (2018.01); C12Q 1/6804 (2018.01); C12Q 1/6809 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12Q 1/6888 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6809 (2013.01)] 30 Claims
 
1. A method of enriching microbial cell-free nucleic acids in a plasma sample obtained from a mammalian host, the method comprising:
(a) providing the plasma sample from the mammalian host, wherein the plasma sample comprises cell-free nucleic acids comprising mammalian host cell-free nucleic acids and the microbial cell-free nucleic acids; and
(b) removing or physically isolating cell-free nucleic acids of one or more length ranges from the plasma sample, thereby enriching the plasma sample for microbial cell-free nucleic acids in the length interval ranges of:
(i) from about 10 to about 60 base pairs,
(ii) from about 240 to about 280 base pairs,
(iii) from about 425 to about 475 base pairs, or
(iv) any combination thereof.