US 12,291,734 B2
Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from C1-substrates
Sean Dennis Simpson, Evanston, IL (US); Michael Koepke, Chicago, IL (US); James Macallister Clomburg, Chicago, IL (US); Juniper Dee Gramling, Skokie, IL (US); Stephanie Rhianon Jones, Evanston, IL (US); Jaeyoung Jung, San Francisco, CA (US); Timothy James Politano, Chicago, IL (US); and Ryan Christopher Tappel, Skokie, IL (US)
Assigned to LanzaTech, Inc., Skokie, IL (US)
Filed by LanzaTech, Inc., Skokie, IL (US)
Filed on Jun. 21, 2023, as Appl. No. 18/339,027.
Claims priority of provisional application 63/366,757, filed on Jun. 21, 2022.
Claims priority of provisional application 63/497,045, filed on Apr. 19, 2023.
Prior Publication US 2024/0026413 A1, Jan. 25, 2024
Int. Cl. C12P 21/02 (2006.01)
CPC C12P 21/02 (2013.01) 7 Claims
 
1. A process for continuous co-production of at least one chemical product and at least one exogenous gene product comprising:
a) providing a continuous bioreactor;
b) introducing to the bioreactor a recombinant C1-fixing microorganism selected from Clostridium autoethanogenum and Cupriavidus necator capable of co-producing at least one chemical product and at least one exogenous gene product, a gaseous substrate comprising one or more of CO, CO2, and H2, and a liquid growth medium;
c) continuously culturing the recombinant C1-fixing microorganism thereby generating a gas fermentation broth comprising 1) the at least one chemical product, 2) the at least one exogenous gene product, and 3) microbial biomass;
d) continuously removing a portion of the gas fermentation broth in a first stream;
e) continuously removing the at least one chemical product in a second stream; and
f) continuously recovering the at least one exogenous gene product from the microbial biomass from the first stream; wherein the at least one exogenous gene product is squid ring teeth (SRT) protein and wherein the at least one chemical product is selected from acetone, ethanol, ethylene, isoprene, and isopropanol.