CPC C12N 9/58 (2013.01) [C12P 7/06 (2013.01); C12Y 304/21 (2013.01)] | 25 Claims |
1. A protease variant comprising a modification at one or more positions corresponding to positions 39, 50, 57, 60, 74, 81, 84, 109, 110, 111, 115, 117, 124, 128, 145, 146, 154, 182, 187, 207, 209, 210, 212, 228, 267, 271, 272, 274, 278, 280, 294, 317, 318, 320, 321, 322, 328, 343, 348, 362 or 363 of the polypeptide of SEQ ID NO: 3, wherein the variant has
(a) protease activity,
(b) at least 90%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 3, and
(c) increased thermostability compared to the protease of SEQ ID NO: 3.
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