	US 12,291,729 B2
	Engineered Cas protein and use thereof
Zhiqiang Duan, Jinan (CN); Ying Chen, Jinan (CN); and Fengming Han, Jinan (CN)
Assigned to SHANDONG SHUNFENG BIOTECHNOLOGY CO.LTD., Jinan (CN)
Filed by SHANDONG SHUNFENG BIOTECHNOLOGY CO.LTD., Jinan (CN)
Filed on Jan. 8, 2024, as Appl. No. 18/406,232.
Application 18/406,232 is a continuation of application No. PCT/CN2023/134558, filed on Nov. 28, 2023.
Claims priority of application No. 202310830438.1 (CN), filed on Jul. 7, 2023; application No. 202310961664.3 (CN), filed on Aug. 1, 2023; application No. 202311078840.5 (CN), filed on Aug. 25, 2023; and application No. 202311230471.7 (CN), filed on Sep. 22, 2023.
Prior Publication US 2025/0011739 A1, Jan. 9, 2025
Int. Cl. C12N 15/00 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/63 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 15/102 (2013.01); C12N 15/111 (2013.01); C12N 15/63 (2013.01); C12N 2310/20 (2017.05)]

15 Claims

1. An engineered type V Cas protein, consisting of a parental type V Cas protein and an HNH domain having nuclease cleavage activity, wherein the parental type V Cas protein comprises SEQ ID NO: 9, and wherein the HNH domain is located between amino acids 794 and 795 of SEQ ID NO: 9.