US 12,291,728 B2
Engineered glycosyltransferases and steviol glycoside glucosylation methods
Jonathan Vroom, South San Francisco, CA (US); Stephanie Sue Galanie, Knoxville, TN (US); Nikki Dellas, San Carlos, CA (US); Jack Liang, South San Francisco, CA (US); Joyce Liu, Fremont, CA (US); David Entwistle, San Carlos, CA (US); and Courtney Dianne Moffett, San Francisco, CA (US)
Assigned to TATE & LYLE SOLUTIONS USA LLC, Hoffman Estates, IL (US)
Filed by TATE & LYLE SOLUTIONS USA LLC, Hoffman Estates, IL (US)
Filed on Oct. 27, 2020, as Appl. No. 17/081,923.
Application 17/081,923 is a division of application No. 15/886,094, filed on Feb. 1, 2018, abandoned.
Claims priority of provisional application 62/479,262, filed on Mar. 30, 2017.
Claims priority of provisional application 62/454,417, filed on Feb. 3, 2017.
Prior Publication US 2022/0306998 A1, Sep. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12P 19/18 (2006.01); C12P 19/56 (2006.01)
CPC C12N 9/1048 (2013.01) [C12N 9/1051 (2013.01); C12N 9/88 (2013.01); C12P 19/18 (2013.01); C12P 19/56 (2013.01); C12Y 204/01 (2013.01); C12Y 204/01013 (2013.01)] 12 Claims
 
1. An engineered glycosyltransferase comprising a polypeptide sequence that has at least 88% sequence identity to SEQ ID NO: 5290 and having glycosyltransferase activity, wherein the polypeptide sequence comprises an amino acid substitution at position 308 of SEQ ID NO: 5290.