	US 12,291,713 B2
	Modified yeast and method for increasing lysine content in fermentation co-products
Jaclyn Diana Demartini, Palo Alto, CA (US); and Celia Emily Gaby Payen, Wilmington, DE (US)
Assigned to DANISCO US INC., Palo Alto, CA (US)
Appl. No. 17/621,982
Filed by DANISCO US INC, Palo Alto, CA (US)
PCT Filed Jun. 22, 2020, PCT No. PCT/US2020/038879 § 371(c)(1), (2) Date Dec. 22, 2021, PCT Pub. No. WO2020/263720, PCT Pub. Date Dec. 30, 2020.
Claims priority of provisional application 62/865,457, filed on Jun. 24, 2019.
Prior Publication US 2022/0251582 A1, Aug. 11, 2022
Int. Cl. C12N 15/81 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01); C12P 13/08 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01)

CPC C12N 15/81 (2013.01) [C12N 9/1025 (2013.01); C12N 15/52 (2013.01); C12P 13/08 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12Y 203/03014 (2013.01)]

9 Claims

1. A non-naturally-occurring variant homocitrate synthase polypeptide having at least 80% amino acid sequence identity to SEQ ID NO: 2 and comprising one or more mutations selected from the group consisting of F36Y, N38K, N289D, R349K, Q352E, V375D, R376T and I380M, with respect to SEQ ID NO: 2, wherein the variant homocitrate synthase polypeptide demonstrates reduced lysine inhibition compared to an otherwise identical homocitrate synthase polypeptide lacking the one or more mutations.