US 12,291,703 B2
Gas concentration adjusting agent for use in culture of bacteria, and method for culturing bacterium using same
Hajime Yamada, Tokyo (JP)
Assigned to MITSUBISHI GAS CHEMICAL COMPANY, INC., Tokyo (JP)
Appl. No. 17/439,080
Filed by MITSUBISHI GAS CHEMICAL COMPANY, INC., Tokyo (JP)
PCT Filed Feb. 20, 2020, PCT No. PCT/JP2020/006689
§ 371(c)(1), (2) Date Sep. 14, 2021,
PCT Pub. No. WO2020/189167, PCT Pub. Date Sep. 24, 2020.
Claims priority of application No. 2019-053078 (JP), filed on Mar. 20, 2019.
Prior Publication US 2022/0154130 A1, May 19, 2022
Int. Cl. C12M 1/34 (2006.01); C12N 1/20 (2006.01)
CPC C12M 41/34 (2013.01) [C12N 1/20 (2013.01)] 5 Claims
 
1. A gas concentration regulator for use in culture of bacteria, comprising: (a) an ascorbic acid component; (b) a transition metal catalyst; (c) activated carbon; (d) an alkali metal carbonate or an alkaline earth metal hydroxide; (e) water; and a thermoplastic resin;
wherein the activated carbon is impregnated with the ascorbic acid component and the water,
the activated carbon is a granular activated carbon having a particle diameter of 0.1 to 2 mm;
an average pore diameter of the activated carbon is 2.7 nm or more and 5.5 nm or less, and
a total pore volume of the activated carbon is 0.60 cm3/g or more and
wherein the thermoplastic resin is polyethylene, polypropylene, or a mixture thereof having a molecular weight of 10,000 or less, and
the thermoplastic resin has a softening point of 90° to 125° C.