US 12,291,701 B2
Cell culture container having minute volume
Yoshiomi Hiroi, Funabashi (JP); Kohei Suzuki, Funabashi (JP); and Natsuki Abe, Shiraoka (JP)
Assigned to NISSAN CHEMICAL CORPORATION, Tokyo (JP)
Appl. No. 16/650,907
Filed by NISSAN CHEMICAL CORPORATION, Tokyo (JP)
PCT Filed Sep. 26, 2018, PCT No. PCT/JP2018/035641
§ 371(c)(1), (2) Date Mar. 26, 2020,
PCT Pub. No. WO2019/065714, PCT Pub. Date Apr. 4, 2019.
Claims priority of application No. 2017-185397 (JP), filed on Sep. 26, 2017.
Prior Publication US 2020/0291339 A1, Sep. 17, 2020
Int. Cl. C12M 1/00 (2006.01); C09D 185/02 (2006.01)
CPC C12M 23/20 (2013.01) [C09D 185/02 (2013.01); C12M 23/22 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A cell culture container comprising at least one opened minute space with a volume of 20 μL or less at a surface of the container, and a hydrophilic coating film containing a copolymer having the recurring units represented by formulae (a1) and (b1):

OG Complex Work Unit Chemistry
wherein Ta, Tb, Ua1, Ua2, Ub1, Ub2 and Ub3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Qa and Qb represent an ester bond, Ra and Rb each independently represent a linear or branched alkylene group having 1 to 18 carbon atoms which may be substituted by halogen atom(s), An− represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, and m represents an integer of 0 to 6,
on the surface portion of the container constituting the minute space,
wherein the hydrophilic coating film has no bubble therein and has a surface with a water contact angle in air of 0° to 120° and a bubble contact angle in water of 80° to 180°, and
wherein the hydrophilic coating film is obtained by coating a varnish containing (i) the copolymer with a solid content of 0.01 to 4% by mass and (ii) water as a solvent.