US 12,291,511 B2
Polymerase inhibitors and related compositions and methods
Wenhui Zhou, Madison, WI (US); Kimberly K. Knoche, Madison, WI (US); Douglas R. Storts, Madison, WI (US); Min Zhou, Madison, WI (US); and Poncho Meisenheimer, Madison, WI (US)
Assigned to Promega Corporation, Madison, WI (US)
Filed by Promega Corporation, Madison, WI (US)
Filed on Feb. 13, 2024, as Appl. No. 18/440,576.
Application 18/049,517 is a division of application No. 16/712,098, filed on Dec. 12, 2019, granted, now 11,524,946, issued on Dec. 13, 2022.
Application 18/440,576 is a continuation of application No. 18/049,517, filed on Oct. 25, 2022, granted, now 11,939,310.
Claims priority of provisional application 62/778,590, filed on Dec. 12, 2018.
Prior Publication US 2024/0182434 A1, Jun. 6, 2024
Int. Cl. C07D 307/42 (2006.01); C07C 317/28 (2006.01); C07D 307/80 (2006.01); C07D 311/58 (2006.01); C12N 9/12 (2006.01); C12P 19/34 (2006.01)
CPC C07D 307/42 (2013.01) [C07C 317/28 (2013.01); C07D 307/80 (2013.01); C07D 311/58 (2013.01); C12N 9/1252 (2013.01); C12P 19/34 (2013.01); C12Y 207/07007 (2013.01)] 20 Claims
 
1. A method of amplifying a nucleic acid comprising:
(a) adding amplification reagents and a nucleic acid template to a DNA polymerase inhibited by a compound of formula (II):

OG Complex Work Unit Chemistry
or a salt thereof,
wherein:
A is selected from a monocyclic or bicyclic aryl, heteroaryl, or heterocyclyl group, each of which may be optionally substituted with 1, 2, or 3 substituents;
R1 is C6-C20 alkyl;
R2 is selected from hydrogen and —COOH;
n is 1 or 2; and
R3 is selected from —COOH and —SO3X, wherein X is selected from hydrogen, an alkali metal cation, and an ammonium cation;
(b) heating to a temperature of at least 80° C. to activate the DNA polymerase; and
(c) running through a thermal cycling protocol of appropriate times and temperatures for the amplification reagents and a nucleic acid template.