US 12,290,813 B2
Apparatus and method for fast digital detection
Kalyan Handique, Ann Arbor, MI (US); Ronald Lebofsky, Kensington, CA (US); and Josh Shinoff, Emeryville, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on May 26, 2021, as Appl. No. 17/330,663.
Claims priority of provisional application 63/030,134, filed on May 26, 2020.
Prior Publication US 2021/0370296 A1, Dec. 2, 2021
Int. Cl. C12Q 1/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6806 (2018.01)
CPC B01L 3/502738 (2013.01) [B01L 7/525 (2013.01); C12Q 1/6806 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0877 (2013.01); B01L 2400/0487 (2013.01)] 20 Claims
OG exemplary drawing
 
19. A method of detecting a target nucleic acid in multiple samples, the method comprising
contacting different samples comprising sample nucleic acids with different pluralities of solid supports to form different mixtures, wherein the solid supports comprise a plurality of capture molecules that bind to the sample target nucleic acid or a vehicle comprising the sample target nucleic acid, optionally wherein the plurality of capture molecules on a solid support are identical;
binding the capture molecules to the sample target nucleic acids, wherein the number of solid supports in the mixture is sufficiently high that at least some solid supports are not bound to target nucleic acids or vehicles comprising target nucleic acids;
introducing the solid supports comprising bound sample target nucleic acids or vehicles comprising sample target nucleic acids into an array of wells, wherein the wells each can contain no more than one solid support, wherein the position of wells receiving the sample beads is recorded such that wells corresponding to different samples is recorded;
detecting in each well (i) the presence or absence of the sample target nucleic acids and (ii) the position of the wells, wherein the detecting is performed with a scanner; and
determining with a computer processor the number of sample target nucleic acids per sample based in part on the number of wells comprising detected sample target nucleic acids, wherein the sample identity is determined by the position of the wells.