	US 12,290,807 B2
	Incubation well with an unvented gas cavity for use in a microfluidic chip
Piotr Garstecki, Warsaw (PL); Jaroslaw Ziólkowski, Konstantynów Lódzki (PL); and Piotr Knap, Hrubieszów (PL)
Assigned to BACTEROMIC SP. Z O.O., Warsaw (PL)
Appl. No. 17/043,449
Filed by BACTEROMIC SP. z O.O., Warsaw (PL)
PCT Filed Mar. 29, 2019, PCT No. PCT/EP2019/058045 § 371(c)(1), (2) Date Sep. 29, 2020, PCT Pub. No. WO2019/185885, PCT Pub. Date Oct. 3, 2019.
Claims priority of application No. 425107 (PL), filed on Mar. 30, 2018; and application No. 18189593 (EP), filed on Aug. 17, 2018.
Prior Publication US 2021/0023553 A1, Jan. 28, 2021
Int. Cl. B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12M 1/32 (2006.01); C12M 3/06 (2006.01)

CPC B01L 3/5027 (2013.01) [C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/22 (2013.01); C12M 23/34 (2013.01); C12M 41/24 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0864 (2013.01)]

25 Claims

1. An incubation segment for use in a microfluidic chip for microbiological assays, wherein said incubation segment is formed in a substrate with a first major face and a second major face, said segment comprises an incubation well, an inlet channel through which a sample may be inputted into said incubation well, and a gas cavity connected to said incubation well by a microfluidic communication channel, wherein said gas cavity is unvented and a base of the gas cavity is connected to a base of the incubation well by the communication channel, wherein the incubation well comprises an optical window, the optical window comprising a through hole extending from said first major face through said substrate to said second major face, a first impermeable, transparent layer covering the incubation well on said first major face, and a second impermeable, transparent layer covering the incubation well on said second major face.