| CPC B01L 3/5023 (2013.01) [B01L 3/5027 (2013.01); G01N 33/54389 (2021.08); B01L 2300/0858 (2013.01); B01L 2300/0861 (2013.01); B01L 2400/082 (2013.01); G01N 33/569 (2013.01)] | 12 Claims |

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1. A device, comprising:
a laminated structure comprising a nitrocellulose substrate layer provided on a hydrophobic support layer,
a plurality of parallel fluid flow channels, each parallel fluid flow channel separated from an adjacent parallel fluid flow channel by a nitrocellulose substrate-devoid gap exposing the hydrophobic support layer, wherein each substrate-devoid gap forms a side channel that is essentially impermeable to flow of an aqueous fluid;
a single sample receiving zone positioned on the substrate to distribute a portion of a sample deposited thereon into each fluid flow channel in the plurality of parallel fluid flow channels;
a fluid control feature ablated or etched on a surface of the nitrocellulose substrate, the fluid control feature composed of a nitrocellulose substrate-devoid area exposing the hydrophobic support layer, wherein the fluid control feature is configured to guide the portion of sample into one or more of the parallel fluid flow channels; and
a capture zone in each of the plurality of fluid flow channels, wherein the capture zone comprises a reagent comprising a binding member.
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