US 12,290,804 B2
Method of treating a sample
Patrick Kinney, Hayward, CA (US); Sujata Iyer, San Jose, CA (US); Philipp Ott, Steg (CH); and Adrian Sager, Mannedorf (CH)
Assigned to TECAN TRADING AG, Mannedorf (CH)
Appl. No. 17/294,853
Filed by TECAN TRADING AG, Mannedorf (CH)
PCT Filed Dec. 20, 2018, PCT No. PCT/US2018/066850
§ 371(c)(1), (2) Date May 18, 2021,
PCT Pub. No. WO2020/131070, PCT Pub. Date Jun. 25, 2020.
Prior Publication US 2022/0023853 A1, Jan. 27, 2022
Int. Cl. B01L 3/02 (2006.01); C12Q 1/6806 (2018.01); G01N 1/28 (2006.01); G01N 1/30 (2006.01); G01N 35/10 (2006.01); G01N 1/31 (2006.01)
CPC B01L 3/0275 (2013.01) [B01L 3/0217 (2013.01); G01N 1/30 (2013.01); B01L 2200/025 (2013.01); B01L 2200/026 (2013.01); G01N 2035/103 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of treating an isolated area of a sample with a liquid, the method comprising the following steps:
generating an isolated area of a sample by means of a first fluid reservoir, the first fluid reservoir enclosing a distal end of a second fluid reservoir, and the isolated area of the sample being sealed towards a remaining area of the sample,
bringing the isolated area of the sample into fluid connection with the second fluid reservoir,
dispensing a partial volume of a fluid from the second fluid reservoir into the first fluid reservoir, thereby generating a fluid flow on the sample at the isolated area in a first direction and moving the fluid onto the sample,
soaking the isolated area of the sample with the partial volume of the fluid for a certain period, whereby during soaking, the fluid contacts the isolated area of the sample without the application of a fluid flow,
dispensing a further partial volume of fluid from the second fluid reservoir into the first fluid reservoir, and soaking the isolated area of the sample for a further time period, and
aspirating the fluid or a partial volume of the fluid from the first fluid reservoir into the second fluid reservoir, thereby generating a fluid flow on the sample at the isolated area in a second direction.