| CPC B01D 15/166 (2013.01) [B01D 15/22 (2013.01); B01F 23/49 (2022.01); B01F 25/31 (2022.01); B01F 35/2132 (2022.01); B01F 35/2133 (2022.01); B01F 35/2211 (2022.01); B01F 35/7176 (2022.01); F04B 23/04 (2013.01); G01N 30/32 (2013.01); G01N 30/34 (2013.01); B01D 15/16 (2013.01); G01N 2030/324 (2013.01); G01N 2030/347 (2013.01); G01N 30/8658 (2013.01)] | 24 Claims |

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11. A chromatography system comprising:
at least one chromatography column;
a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution, wherein the T-joint receives the second solution from a solution supply unit connected to the T-joint, wherein the T-joint is connected to the solution supply unit using at least one direct connection tube, and wherein the T-joint is connected through at least one valve to a further solution supply unit for supplying the at least one first solution;
at least one low pressure pump for supplying the at least one first solution into the T-joint; and
a high pressure pump for collecting and delivering the buffer solution to the at least one chromatography column,
the chromatography system being characterized in that said T-joint includes a configuration for mixing the at least one first solution and the second solution, wherein the configuration for mixing the at least one first solution and the second solution is a magnetic stirrer,
wherein the system is configured such that the low-pressure pump, the T-joint, and the magnetic stirrer operate at a pressure of less than 0.7 mega Pascals (MPa).
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