	US 12,290,577 B2
	Method of freeze drying surfactant-stabilized microbubbles
Brian Edward Oeffinger, Philadelphia, PA (US); Margaret A. Wheatley, Media, PA (US); Rawan Shraim, Philadelphia, PA (US); Purva Vaidya, Flushing, NY (US); and John Robert Eisenbrey, Wayne, PA (US)
Assigned to Thomas Jefferson University, Philadelphia, PA (US); and Drexel University, Philadelphia, PA (US)
Filed by Thomas Jefferson University, Philadelphia, PA (US); and Drexel University, Philadelphia, PA (US)
Filed on Jun. 30, 2022, as Appl. No. 17/810,157.
Application 17/810,157 is a division of application No. 16/817,898, filed on Mar. 13, 2020, granted, now 11,389,552.
Claims priority of provisional application 62/817,886, filed on Mar. 13, 2019.
Prior Publication US 2023/0044615 A1, Feb. 9, 2023
Int. Cl. A61K 49/22 (2006.01)

CPC A61K 49/223 (2013.01) [A61K 49/227 (2013.01); A61K 2800/84 (2013.01)]

6 Claims

1. A method for annealing surfactant-stabilized microbubbles, the method comprising:

preparing vials comprising a mixture comprising surfactant-stabilized microbubbles;

passing the vials in and out of liquid nitrogen (LN₂) until the mixture is frozen;

holding the vials at −20° C.;

placing the vials on a cooled shelf of a lyophilizer, wherein the cooled shelf has a temperature between about −10° C. to about −30° C.;

freeze-drying the vials in the lyophilizer; and

capping the freeze-dried vials.