| CPC A61K 39/245 (2013.01) [A61P 31/20 (2018.01); C12N 7/00 (2013.01); A61K 2039/552 (2013.01)] | 4 Claims |

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1. A method of preventing or treating congenital infection with HCMV in a subject, comprising administering a vaccine which comprises a modified human cytomegalovirus (HCMV) envelope glycoprotein B (gB protein) antigen and a pentamer antigen consisting of HCMV gH, gL, UL128, UL130, and UL131 to the subject, wherein the gB protein antigen is an HCMV gB protein ectodomain variant comprising the amino acid substitutions at positions 156, 157, 239, 240, 456, and 458 in the amino acid sequence set forth in SEQ ID NO:7.
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