US 12,290,074 B2
Agricultural compositions comprising remodeled nitrogen fixing microbes
Mark Reisinger, Berkeley, CA (US); Ernest Sanders, Berkeley, CA (US); Richard Broglie, Berkeley, CA (US); and Karsten Temme, Berkeley, CA (US)
Assigned to Pivot Bio, Inc., Berkeley, CA (US)
Filed by Pivot Bio, Inc., Berkeley, CA (US)
Filed on Apr. 16, 2024, as Appl. No. 18/636,988.
Application 18/636,988 is a continuation of application No. 18/540,318, filed on Dec. 14, 2023.
Application 18/540,318 is a continuation of application No. 17/254,175, granted, now 11,963,530, previously published as PCT/US2019/039217, filed on Jun. 26, 2019.
Claims priority of provisional application 62/808,693, filed on Feb. 21, 2019.
Claims priority of provisional application 62/690,621, filed on Jun. 27, 2018.
Prior Publication US 2024/0298647 A1, Sep. 12, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 63/20 (2020.01)
CPC A01N 63/20 (2020.01) 30 Claims
 
1. A composition, comprising:
a) a plurality of non-intergeneric remodeled bacteria; and
b) at least one fertilizer;
wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
wherein the non-intergeneric remodeled bacteria have an average colonization ability per unit of plant root tissue of at least about 1.0×104 bacterial cells per gram of fresh weight of plant root tissue; and
wherein the at least one fertilizer comprises one or more of ammonia, glutamine, urea, and a nitrate; or
wherein the at least one fertilizer comprises one or more of nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, boron, chloride, manganese, iron, zinc, copper, molybdenum, selenium, and any salt thereof; or
wherein the at least one fertilizer comprises one or more of an amino acid, a salt, a vitamin, glucose, sodium chloride, a yeast extract, ammonium dihydrogen phosphate, ammonium sulfate, glycerol, lactic acid, propionic acid, succinic acid, malic acid, citric acid, potassium bitartrate, xylose, lyxose, and lecithin; or
wherein the at least one fertilizer comprises one or more of a nitrification inhibitor, a nitrogen stabilizer, and a urease inhibitor.