	US 12,290,068 B2
	Single cell encapsulation via pickering emulsion for bio-pesticides application
Guy Mechrez, Rishon Lezion (IL); and Dana Ment, Rishon Lezion (IL)
Assigned to The State of Israel, Ministry of Agriculture & Rural Development Agricultural Research Organization, Rishon Lezion (IL)
Appl. No. 17/059,233
Filed by The State of Israel, Ministry of Agriculture & Rural Development Agricultural Research Organization (ARO), Rishon Lezion (IL)
PCT Filed May 27, 2019, PCT No. PCT/IB2019/054370 § 371(c)(1), (2) Date Nov. 26, 2020, PCT Pub. No. WO2019/229624, PCT Pub. Date Dec. 5, 2019.
Claims priority of provisional application 62/676,984, filed on May 27, 2018.
Prior Publication US 2021/0212317 A1, Jul. 15, 2021
Int. Cl. A01N 25/28 (2006.01); C12N 11/04 (2006.01); C12N 11/14 (2006.01)

CPC A01N 25/28 (2013.01) [C12N 11/04 (2013.01); C12N 11/14 (2013.01)]

7 Claims

1. A method for single cell encapsulation comprising:

providing a water-based solution including amine functionalized silica nanoparticles;

emulsifying the water-based solution by adding an oil solution into the water-based solution to form oil-in-water Pickering emulsions;

adding cells of Metarhizium spore into said solution containing the oil-in-water Pickering emulsions;

generating at least one single cell encapsulation of the cells by vortexing, wherein the content of the nanoparticles is between 0.1 wt % to 10 wt %, and the oil/water ratio is one of: 1:99, 5:95, 10:90, 20:80, 30:70, 40:60 and 50:50 respectively.