US 11,971,417 B2
Single-molecule protein and peptide sequencing
Daniel Masao Estandian, Cambridge, MA (US); Alexi Georges Choueiri, Chandler, AZ (US); Edward Stuart Boyden, Chestnut Hill, MA (US); and Asmamaw Wassie, Boston, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Oct. 12, 2022, as Appl. No. 18/046,012.
Application 18/046,012 is a continuation of application No. 16/736,248, filed on Jan. 7, 2020, granted, now 11,499,979.
Claims priority of provisional application 62/789,850, filed on Jan. 8, 2019.
Prior Publication US 2023/0305017 A1, Sep. 28, 2023
Int. Cl. G01N 33/68 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/6818 (2013.01) [G01N 33/58 (2013.01); G01N 2458/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for processing a terminal amino acid of a peptide comprising:
(a) contacting the peptide, immobilized to a substrate, with a compound comprising a substrate-tethering group, wherein the compound binds to the terminal amino acid or a derivative of the terminal amino acid to form a compound-peptide complex;
(b) tethering the compound-peptide complex to the substrate via the substrate-tethering group while the peptide is immobilized to the substrate; and
(c) subsequent to (b), cleaving the terminal amino acid or the derivative of the terminal amino acid from the peptide, thereby providing a compound-terminal amino acid complex tethered to the substrate.