US 11,971,401 B2
Method for rapid identification of pharmacologically active chemical entities associated with the efficacy of ethnobotanical substances
Mangala P. Bajpai, Simi Valley, CA (US)
Assigned to Biological Life, Inc, Simi Valley, CA (US)
Filed by Biological Life, Inc., Simi Valley, CA (US)
Filed on Nov. 22, 2019, as Appl. No. 16/692,727.
Application 16/692,727 is a division of application No. 12/054,129, filed on Mar. 24, 2008, abandoned.
Prior Publication US 2020/0088720 A1, Mar. 19, 2020
Int. Cl. G01N 33/50 (2006.01); C12Q 1/00 (2006.01); G01N 33/94 (2006.01)
CPC G01N 33/5067 (2013.01) [C12Q 1/00 (2013.01); G01N 33/5038 (2013.01); G01N 33/5044 (2013.01); G01N 33/94 (2013.01)] 18 Claims
 
1. A method for identifying medicinally active chemical entities in ethnobotanical substances, comprising the steps of:
(1) providing an ethnobotanical natural substance occurring naturally in nature, the ethnobotanical substance comprising an existing land or marine plant source identified as having a previously unidentified active agent associated with a medicinal effect on humans in the form of previously unidentified medicinally active chemical entities which are the product of bodily function which occurs when the ethnobotanical substance is ingested by the human user, wherein the land or marine plant source consists of roots or fruits or seeds or bark or leaves;
(2) performing an in-vitro assay with the ethnobotanical substance with one or more of the following: (a) human intestinal preparations and (b) human liver preparations, thereby producing an array of human chemical entities;
(3) performing an in-vitro assay of said ethnobotanical substance using one or more of the following: (a) animal intestinal preparations and (b) animal liver preparations from at least a plurality of animal species to produce an array of animal chemical entities;
(4) performing an in-vivo dosing of the ethnobotanical substance with an animal species from the plurality of animal species having animal chemical entities which match the human chemical entities;
(5) obtaining a biological fluid sample from the animal species of (4) following the in-vivo dosing thereof;
(6) performing an analysis of the biological fluid sample of (5) to determine the presence of chemical entities in the biological fluid sample; and
(7) wherein any matches between the biological fluid sample chemical entities of the animal species of (5) and the human chemical entities from the in-vitro assay are potentially medicinally active chemical entities associated with the efficacy of the ethnobotanical substance.