|
1. A computer-implemented method comprising: repeatedly calculating a time-of-fight of acoustic signals traversing through a biological specimen immersed in a cross-linking fixative solution until the calculated time-of-flight reaches a predetermined threshold value, wherein the cross-linking fixative solution is maintained at a temperature ranging from between about 0° C. to about 15° C. while the time-of-flight is repeatedly calculated, wherein the calculating of the time-of-flight comprises: (i) measuring a triangle wave using a frequency sweep of the acoustic signal traversing through the biological tissue specimen immersed in the cross-linking fixative solution; (ii) comparing the measured triangle wave with a plurality of candidate triangle waves, wherein the plurality of candidate triangle waves are generated over a plurality of candidate frequencies and over a plurality of candidate phases; and (iii) identifying a candidate triangle wave of the plurality of triangle waves that is a closest-match to the measured triangle wave.
|
