US 11,970,785 B2
Methods and materials for multiplexed collections of functional ligands
George W. Jackson, Pearland, TX (US); Robert Batchelor, Pearland, TX (US); Alexander S. Chiu, Pearland, TX (US); Rafal Drabek, Houston, TX (US); Deepak Thirunavukarasu, Houston, TX (US); and Caitlin Bruns, Webster, TX (US)
Assigned to BASE PAIR BIOTECHNOLOGIES, INC., Pearland, TX (US)
Filed by Base Pair Biotechnologies, Inc., Pearland, TX (US)
Filed on Aug. 30, 2021, as Appl. No. 17/460,513.
Application 17/460,513 is a division of application No. 16/262,825, filed on Jan. 30, 2019, granted, now 11,111,603.
Claims priority of provisional application 62/624,063, filed on Jan. 30, 2018.
Prior Publication US 2021/0395920 A1, Dec. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6837 (2018.01); C40B 40/06 (2006.01)
CPC C40B 40/06 (2013.01) [C12Q 1/6811 (2013.01); C12Q 1/6837 (2013.01)] 18 Claims
 
1. A method for utilizing a nucleic acid array comprising:
providing a substrate with a plurality of single-stranded nucleic acid ligands located and attached at predetermined addresses on said substrate, each of said single-stranded nucleic acid ligands being an aptamer binding to a known target molecule with an identical hybridization sequence for each of said single-stranded nucleic acid ligands at one of said predetermined addresses, and forming a hybridization to a single-stranded nucleic acid label, each comprising a complementary hybridization sequence to said hybridization sequence;
incubating said substrate with a sample which may potentially contain at least one target molecule which binds to at least one of said plurality of single-stranded nucleic acid ligands; and
determining if any changes in said hybridizations occur after said incubating by performing a detection operation to detect potential changes in said hybridizations between said single-stranded nucleic acid labels and said single-stranded nucleic acid ligands correlated to said addresses;
wherein said potential changes in said hybridizations indicate binding of said at least one target molecule to at least one of said plurality of single-stranded nucleic acid ligands when a change in said hybridization occurs after adding said sample and no change in said hybridization after adding said sample indicates no binding of said at least one target molecule to any of said plurality of single-stranded nucleic acid ligands.