US 11,970,739 B2
Multiplex capture of gene and protein expression from a biological sample
Jennifer Chew, Hayward, CA (US); Joseph Francis Shuga, Pleasanton, CA (US); Patrick Roelli, Stockholm (SE); Denise Cheung, Dublin, CA (US); Alexander Hermes, Livermore, CA (US); Yifeng Yin, Elk Grove, CA (US); Naishitha Anaparthy, Dublin, CA (US); Ryo Hatori, Pleasanton, CA (US); Marlon Stoeckius, Stockholm (SE); Rapolas Spalinskas, Pleasanton, CA (US); Anna-Maria Katsori, Stockholm (SE); Cedric Uytingco, Milpitas, CA (US); Mesruh Turkekul, Stockholm (SE); David Sukovich, Oakland, CA (US); Christina Galonska, Stockholm (SE); Aleksandra Jurek, Pleasanton, CA (US); and Octavian Marian Bloju, Stockholm (SE)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Jul. 6, 2023, as Appl. No. 18/348,063.
Application 18/348,063 is a continuation of application No. 17/867,223, filed on Jul. 18, 2022, granted, now 11,739,381.
Application 17/867,223 is a continuation of application No. PCT/US2022/020985, filed on Mar. 18, 2022.
Claims priority of provisional application 63/311,703, filed on Feb. 18, 2022.
Claims priority of provisional application 63/270,230, filed on Oct. 21, 2021.
Claims priority of provisional application 63/252,335, filed on Oct. 5, 2021.
Claims priority of provisional application 63/245,697, filed on Sep. 17, 2021.
Claims priority of provisional application 63/214,058, filed on Jun. 23, 2021.
Claims priority of provisional application 63/162,870, filed on Mar. 18, 2021.
Prior Publication US 2024/0011090 A1, Jan. 11, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); C12N 15/10 (2006.01); C12Q 1/6837 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6837 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A composition comprising:
a) a spatial array comprising a substrate comprising:
(i) a first plurality of capture probes wherein the first plurality of capture probes comprise a spatial barcode and a first plurality of capture domains; and
(ii) a second plurality of capture probes wherein the second plurality of capture probes comprise a spatial barcode and a second plurality of capture domains hybridized to a plurality of oligonucleotides from an analyte capture agent, wherein the analyte capture agent comprises an analyte-binding moiety comprising an antibody or an antigen-binding fragment thereof, and wherein the oligonucleotides comprise an analyte capture sequence and an analyte binding moiety barcode; and
b) a pair of probes comprising:
(i) a plurality of first probes, wherein a first probe of the plurality of first probes is hybridized to a target nucleic acid and
(ii) a plurality of second probes, wherein a second probe of the plurality of second probes is hybridized to the target nucleic acid at a separate location from the first probe and wherein the second probe has a sequence complementary to the first plurality of capture domains.