US 11,970,725 B2
Reduced genome E. coli lacking toxin-antitoxin genes
Frederick R. Blattner, Madison, WI (US); Robert E. Novy, Verona, WI (US); David A. Frisch, Fitchburg, WI (US); Charles Landry, Fitchburg, WI (US); Hyunsic Choi, Madison, WI (US); Eric A. Steffen, Mount Horeb, WI (US); and John Brandon, Madison, WI (US)
Assigned to Scarab Genomics LLC, Madison, WI (US)
Filed by SCARAB GENOMICS LLC, Madison, WI (US)
Filed on Apr. 12, 2023, as Appl. No. 18/299,479.
Application 18/299,479 is a division of application No. 16/644,444, granted, now 11,667,944, previously published as PCT/US2018/049422, filed on Sep. 4, 2018.
Claims priority of provisional application 62/554,443, filed on Sep. 5, 2017.
Prior Publication US 2023/0287472 A1, Sep. 14, 2023
Int. Cl. C12N 1/21 (2006.01); C07K 14/21 (2006.01); C07K 14/285 (2006.01); C07K 14/34 (2006.01); C12N 1/20 (2006.01); C12N 9/18 (2006.01); C12P 21/06 (2006.01)
CPC C12P 21/06 (2013.01) [C07K 14/21 (2013.01); C07K 14/285 (2013.01); C07K 14/34 (2013.01); C12N 1/20 (2013.01); C12N 9/18 (2013.01); C07K 2319/02 (2013.01)] 13 Claims
 
1. A non-naturally occurring E. coli bacterium having a genome between 4.41 Mb and 2.78 Mb and lacking at least the following toxin-antitoxin genes: yafQ, dinJ, hha, tomB, gnsA, ymcE, yoeB, yefM, mazF, mazE, mazG, cptA/ygfX, cptB/sdhE, mqsR, mqsA, higB, higA, yhaV, prlF, ldrD, rdlD, istR-2, tisB, chpB, chpS, ratA, ratB, ldrA, rdlA, ldrB, rdlB, ldrC, rdlC, hokB, sokB, sibA, ibsA, sibB, ibsB, ohsC, shoB, sibC, ibsC, sibD, sibE, ibsD, ibsE, dinQ, agrA, agrB, ghoT, ghoS, yfeC, yfeD, fic, yhfG, yhjJ, yhjM, yhjN, yjjJ, ecnA, and ecnB.