US 11,970,690 B2
Methods of cultivating Bordetella species
Patrick Farrell, Holland Landing (CA); Bo Zhi Sun, Waterdown (CA); Fabien Barbirato, Brignais (FR); Javier de Jesus Menendez Diaz, Pickering (CA); and Andrew Chiappetta, Vaughan (CA)
Assigned to SANOFI PASTEUR INC., Swiftwater, PA (US)
Appl. No. 16/981,200
Filed by SANOFI PASTEUR INC., Swiftwater, PA (US)
PCT Filed Mar. 19, 2019, PCT No. PCT/IB2019/000305
§ 371(c)(1), (2) Date Sep. 15, 2020,
PCT Pub. No. WO2019/180507, PCT Pub. Date Sep. 26, 2019.
Claims priority of provisional application 62/645,473, filed on Mar. 20, 2018.
Prior Publication US 2020/0407678 A1, Dec. 31, 2020
Int. Cl. C12N 1/20 (2006.01); A61K 39/02 (2006.01); C12N 1/38 (2006.01)
CPC C12N 1/20 (2013.01) [A61K 39/099 (2013.01); C12N 1/38 (2013.01); C12N 2500/02 (2013.01); C12N 2500/34 (2013.01); C12N 2500/60 (2013.01); C12N 2500/72 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for cultivating a species of Bordetella, which method comprises:
cultivating a Bordetella species under aerobic conditions in a liquid culture medium; and
maintaining a pH of the liquid culture medium during cultivation of said species, wherein maintaining the pH of the liquid culture medium comprises:
adding a first and second acid to the liquid culture medium to maintain the pH of the liquid culture medium within a predetermined range of pH values during cultivation of said Bordetella species, wherein said first acid is nitric acid, hydrochloric acid or sulfuric acid and said second acid is phosphoric acid.